Oxidative activity of corpus luteum and ovarian parenchyma in Bos taurus indicus heifers

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چکیده

The aim of this study was to evaluate the oxidative stress in ovaries and corpus luteum (CL) Bos taurus indicus females oxidant effect CL ovarian tissues regions near, intermediate, or distant from it. Ovaries (n=12) Nelore heifers (n=6) were collected a slaughterhouse fragmented. Experiment 1, each ovary obtained three fragments, resulting 18 fragments with (OV+CL) another without (OV-CL). Three generated CL, totaling fragments. In experiment 2, removed specific CL. All placed Eppendorf-type microtubes (1 mL), kept thermal container at 4 ºC, then stored -80 ºC freezer for analysis (TBARS NBT) antioxidant potential (FRAP ABTS). activity analysis, luteal showed more than tissue = P < 0.0001; ABTS 0.02). had lower levels reactive oxygen species (ROS; TBARS 0.03; NBT 0.0001) tissues. There no difference between different (OV+CL versus OV-CL; > 0.05). presence did not influence gonad. Thus, removal indicates that biotechnologies such as vitro follicle cultivation is possible.

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ژورنال

عنوان ژورنال: Animal reproduction

سال: 2023

ISSN: ['1984-3143', '1806-9614']

DOI: https://doi.org/10.1590/1984-3143-ar2022-0084